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Briggs agar

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DOI: 10.1016/s0079-6352(05)80025-6

Abstract

Publisher Summary This chapter discusses that Briggs tomato juice agar is developed primarily for the cultivation of lactobacilli from milk and dairy products and has since been successfully used for strains from other sources. It is used for the non-selective enumeration of the whole group of lactic acid bacteria and has better productivity for some strains of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus salivarius ssp., Thermophilus and Lactococcus lactis ssp. lactis than MRS agar. Briggs agar is composed of tryptone, peptone, yeast extract, glucose, starch, soluble, sorbitan monooleate, sodium chloride, and agar. The chapter reviews that Briggs agar is an elective medium that gives good colony counts and a similar colony size and appearance for all lactic acid bacteria. Other microorganisms may be distinguished by pigments and extraordinary colonial morphology. Confirmation tests example, gram stain, catalase reaction, are necessary to distinguish mixed microflora. A selective medium for lactobacilli or lactic acid bacteria should be used in parallel where very mixed microfloras are present. It also discusses Briggs agar quality assessment, which is done by its productivity and characteristic appearance of colonies.

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