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Peptide mapping on cellulose thin layers

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
30
Identifiers
DOI: 10.1016/s0021-9673(00)84179-5
Disciplines
  • Biology

Abstract

Abstract A method is describe which uses electrophoresis followed by chromatography on cellulose thin layers to achieve separation of peptides resulting from tryptic digestion of protein. Tryptic digests of 5 proteins were tested: glyceraldehyde-3-phosphate dehydrogenase, TYMV coat protein, canine haemoglobin, R 17 bactriophage coat protein adn ribonuclease. The application of 50–100 μg of diget to a band origin is sufficient to give a good colour development of peptide spots with ninhydrin. More spots were found in all digests than was anticipated from the number of lysine and arginine residues in the proteins.

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