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The C type lectins DC-SIGN and L-SIGN: receptors for viral glycoproteins.

Authors
  • Lozach, Pierre-Yves
  • Burleigh, Laura
  • Staropoli, Isabelle
  • Ali Amara
Type
Book
Journal
Methods in Molecular Biology
Publication Date
Jun 16, 2007
Volume
379
Pages
51–68
Identifiers
DOI: 10.1007/978-1-59745-393-6_4
PMID: 17502670
Source
USPC - SET - SVS
License
Green

Abstract

DC-SIGN and L-SIGN are C-type lectins that recognize carbohydrate structures present on viral glycoproteins and function as attachment factors for several enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (cis-infection). They are also able to capture viruses and transfer viral infections to other target cells (trans-infection). In this chapter, we will give an overview of protocols used to produce soluble viral glycoproteins at high levels and to study the molecular basis of viruses/DC-SIGN and L-SIGN binding and internalization. We will also describe techniques to investigate the molecular mechanisms by which DC-SIGN or L-SIGN spread viral infections.

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