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C-terminal sequencing of peptides using electrospray ionization mass spectrometry.

Authors
  • Rosnack, K J
  • Stroh, J G
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Nov 01, 1992
Volume
6
Issue
11
Pages
637–640
Identifiers
PMID: 1467548
Source
Medline
License
Unknown

Abstract

A low-flow reactor is described for the on-line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16,951.5). Both peptides gave interpretable results. The first 19 amino acids of glucagon were successfully sequenced. Apomyoglobin yielded sequence information to the 30th amino acid with some gaps. At 300 nL/min, 50% of the first 30 amino acids were sequenced and at 1 microL/min, 67% of the first 30 amino acids were observed.

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