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Bulk double emulsification for flow cytometric analysis of microfluidic droplets.

Authors
  • Sukovich, David J1
  • Kim, Samuel C
  • Ahmed, Noorsher
  • Abate, Adam R
  • 1 Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158, USA. [email protected]
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Dec 04, 2017
Volume
142
Issue
24
Pages
4618–4622
Identifiers
DOI: 10.1039/c7an01695f
PMID: 29131209
Source
Medline
License
Unknown

Abstract

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

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