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Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.

Authors
  • Creech, Amanda L
  • Taylor, Jordan E
  • Maier, Verena K
  • Wu, Xiaoyun
  • Feeney, Caitlin M
  • Udeshi, Namrata D
  • Peach, Sally E
  • Boehm, Jesse S
  • Lee, Jeannie T
  • Carr, Steven A
  • Jaffe, Jacob D
Type
Published Article
Journal
Methods
Publisher
Elsevier
Publication Date
Jan 15, 2015
Volume
72
Pages
57–64
Identifiers
DOI: 10.1016/j.ymeth.2014.10.033
PMID: 25448295
Source
Medline
Keywords
License
Unknown

Abstract

Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.

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