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Buccal micronucleus cytome assay: Inter-laboratory scoring exercise and micronucleus and nuclear abnormalities frequencies in different populations from Brazil.

Authors
  • Rohr, Paula1
  • da Silva, Gabrieli Flesch2
  • Vicentini, Veronica Elisa Pimenta3
  • Almeida, Igor Vivian de3
  • Dos Santos, Raquel Alves4
  • Takahashi, Catarina Satie5
  • Goulart, Mirian Oliveira4
  • da Silva, Glenda Nicioli6
  • de Oliveira, Luiza Barbosa6
  • Grisolia, Cesar K7
  • Piau, Tathyana B7
  • Bassi Branco, Carmen Lucia8
  • Reis, Érica de Melo8
  • de Oliveira Galvão, Marcos Felipe9
  • de Medeiros, Silvia R Batistuzzo9
  • Monteiro, Magaly Sales10
  • de Vasconcelos Lopes, Reynaldo Assis10
  • Brandão, Sabrina Fuziger Inácio10
  • Batista, Nelson Jorge Carvalho11
  • Paz, Márcia Fernanda Correia Jardim12
  • And 1 more
  • 1 Laboratório de Genética Toxicológica, Programa de Pós- Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil. Electronic address: [email protected] , (Brazil)
  • 2 Laboratório de Genética Toxicológica, Programa de Pós- Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil. , (Brazil)
  • 3 Laboratório de Mutagênese e Monitoramento Ambiental, Universidade Estadual de Maringá, UEM, Maringá, PR, Brazil. , (Brazil)
  • 4 Laboratório de Genética e Biologia Molecular, Universidade de Franca, UNIFRAN, Franca, SP, Brazil. , (Brazil)
  • 5 Departmento de Genética, Escola de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil; Departmento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. , (Brazil)
  • 6 Laboratório de Pesquisas Clínicas, Universidade Federal de Ouro Preto, UFOP, Ouro Preto, MG, Brazil. , (Brazil)
  • 7 Laboratório de Genética Toxicológica Instituto de Biologia, Universidade de Brasilia, UnB, Brasília, DF, Brazil. , (Brazil)
  • 8 Laboratório de Mutagênese, Universidade Federal de Mato Grosso, UFMT, Cuiabá, MT, Brazil. , (Brazil)
  • 9 Laboratório de Mutagênese Ambiental, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. , (Brazil)
  • 10 Núcleo Bioprospecção e Experimentação Molecular-NUBEM, Centro Universitário INTA-UNINTA, Sobral, CE, Brazil. , (Brazil)
  • 11 Laboratório de Genética Toxicológica, Centro Universitário Santo Agostinho, Terezina, PI, Brazil. , (Brazil)
  • 12 Laboratório de Pesquisa em Genética Toxicológica, Universidade Federal do Piauí, UFPI, Terezina, PI, Brazil. , (Brazil)
  • 13 Laboratório de Genética Toxicológica, Programa de Pós- Graduação em Biologia Celular e Molecular Aplicada à Saúde (PPGBioSaúde), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil; Laboratório de Genética Toxicológica, Programa de Pós- Graduação Profissional em Saúde e Desenvolvimento Humano (PPGSDH), Universidade La Salle, UniLaSalle, Canoas, RS, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Toxicology letters
Publication Date
Aug 22, 2020
Volume
333
Pages
242–250
Identifiers
DOI: 10.1016/j.toxlet.2020.08.011
PMID: 32841739
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The Buccal Micronucleus Cytome Assay (BMCyt) has become an important biomonitoring tool for assessing cytogenetic damage in many studied populations. Each laboratory applies protocols that vary according to the method of collecting and preparing samples. Besides, Brazil is a country of great territorial extensions that received immigrants from various parts of the world with different genetic backgrounds. Therefore, the present study aimed to evaluate the inter-laboratory variation in scoring the same set of slides using the more comprehensive scoring criteria, to standardize the BMCyt protocol, to observe the basal alterations in populations of different Brazilian regions and to compare it with other places around the world. Our results showed that a valuable number of laboratories participated, ten laboratories from different regions of the country, for the validation of the BMCyt in human biomonitoring studies, resulting in the 804 healthy individuals. This was possible because we observed: a range of measures needs to be considered, such as the baseline frequency of DNA damage and cell death in non-exposed individuals; age when grouped showed an influence on DNA damage, although when evaluated by group we did not see an influence; association between smoking habit and all endpoints of the BMCyt (except karyolytic cells) was evident; the basal MN frequency, in the majority of groups, follows those around the world; and the BMCyt was confirmed as a good health status biomarker. We emphasize the need for constant discussions on the parameters of cell death due to greater difficulty among the analyzers. Copyright © 2020 Elsevier B.V. All rights reserved.

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