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B-type esterases in the snail <em>Xeropicta derbentina</em>: an enzymological analysis to evaluate their use as biomarkers of pesticide exposure

Authors
  • Laguerre, Christel
  • Sanchez-Hernandez, Juan C.
  • Köhler, Heinz-R.
  • Triebskorn, Rita
  • Capowiez, Yvan
  • Rault, Magali
  • Mazzia, Christophe
Publication Date
Jan 01, 2009
Identifiers
DOI: 10.1016/j.envpol.2008.07.003
OAI: oai:HAL:hal-02665833v1
Source
HAL-Descartes
Keywords
Language
English
License
Unknown
External links

Abstract

The study was prompted to characterize the B-type esterase activities in the terrestrial snail <em>Xeropicta derbentina</em> and to evaluate its sensitivity to organophosphorus and carbamate pesticides. Specific cholinesterase and carboxylesterase activities were mainly obtained with acetylthiocholine (Km =77.2 mM; Vmax= 38.2 mU/mg protein) and 1-naphthyl acetate (Km= 222 mM, Vmax= 1095 mU/mg protein) substrates, respectively. Acetylcholinesterase activity was concentration-dependently inhibited by chlorpyrifos-oxon, dichlorvos, carbaryl and carbofuran (IC50 =1.35 x 105–3.80 x 108 M). The organophosphate-inhibited acetylcholinesterase activity was reactivated in the presence of pyridine-2- aldoxime methochloride. Carboxylesterase activity was inhibited by organophosphorus insecticides (IC50 =1.20 x 105–2.98 x 108 M) but not by carbamates. B-esterase-specific differences in the inhibition by organophosphates and carbamates are discussed with respect to the buffering capacity of the carboxylesterase to reduce pesticide toxicity. These results suggest that B-type esterases in<em> X. derbentina</em> are suitable biomarkers of pesticide exposure and that this snail could be used as sentinel species in field monitoring of Mediterranean climate regions

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