Affordable Access

Brugia malayi abundant larval transcript 2 protein treatment attenuates experimentally-induced colitis in mice.

Authors
  • Khatri, Vishal
  • Amdare, Nitin
  • Yadav, Ravi Shankar
  • Tarnekar, Aaditya
  • Goswami, Kalyan
  • Reddy, Maryada Venkata Rami
Type
Published Article
Journal
Indian journal of experimental biology
Publication Date
Nov 01, 2015
Volume
53
Issue
11
Pages
732–739
Identifiers
PMID: 26669016
Source
Medline
Language
English
License
Unknown

Abstract

Helminths are known to modulate host's immunity by suppressing host protective pro-inflammatory responses. Such immunomodulatory effects have been experimentally shown to have therapeutic implications in immune mediated disorders. In the present study, we have explored a filarial protein i.e. Brugia malayi recombinant abundant larval transcript 2 (rBmALT2) for its therapeutic effect in dextran sodium sulfate (DSS) induced colitis in mouse model. The immunomodulatory activity of rBmALT-2 was initially confirmed by demonstrating that it suppressed the lipopolysaccharide (LPS) induced nitric oxide synthesis and down-regulated the expression of pro-inflammatory cytokines in vitro by peritoneal exudate cells of mice. Treatment with rBmALT2 reduced severity of colitis associated with significant reduction in weight loss, disease activity, colon damage, mucosal edema and histopathological score including myeloperoxidase activity in colon tissues. rBmALT2 was comparatively more effective in attenuation of colitis when used in the preventive mode than when used for curative purpose. The therapeutic effect of rBmALT2 was found to be associated with downregulation of IFN-γ, IL-6, IL-17 and upregulation of IL-10 cytokines. These results provide strong experimental evidence that BmALT2 could be a potential alternative therapeutic agent in colitis.

Report this publication

Statistics

Seen <100 times