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Bromodomain proteins GTE9 and GTE11 are essential for specific BT2-mediated sugar and ABA responses in Arabidopsis thaliana.

Authors
  • Misra, Anjali1
  • McKnight, Thomas D1
  • Mandadi, Kranthi K2
  • 1 Department of Biology, Texas A&M University, 3258 TAMU, College Station, TX, 77843-3258, USA.
  • 2 Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research & Extension Center, The Texas A&M University System, 2415 East Highway 83, Weslaco, TX, 78596-8344, USA. [email protected]
Type
Published Article
Journal
Plant molecular biology
Publication Date
Mar 01, 2018
Volume
96
Issue
4-5
Pages
393–402
Identifiers
DOI: 10.1007/s11103-018-0704-2
PMID: 29363002
Source
Medline
Keywords
License
Unknown

Abstract

Global Transcription Factor Group E proteins GTE9 and GTE11 interact with BT2 to mediate ABA and sugar responses in Arabidopsis thaliana. BT2 is a BTB-domain protein that regulates responses to various hormone, stress and metabolic conditions in Arabidopsis thaliana. Loss of BT2 results in plants that are hypersensitive to inhibition of germination by abscisic acid (ABA) and sugars. Conversely, overexpression of BT2 results in resistance to ABA and sugars. Here, we report the roles of BT2-interacting partners GTE9 and GTE11, bromodomain and extraterminal-domain proteins of Global Transcription Factor Group E, in BT2-mediated responses to sugars and hormones. Loss-of-function mutants, gte9-1 and gte11-1, mimicked the bt2-1-null mutant responses; germination of all three mutants was hypersensitive to inhibition by glucose and ABA. Loss of either GTE9 or GTE11 in a BT2 over-expressing line blocked resistance to sugars and ABA, indicating that both GTE9 and GTE11 were required for BT2 function. Co-immunoprecipitation of BT2 and GTE9 suggested that these proteins physically interact in vivo, and presumably function together to mediate responses to ABA and sugar signals.

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