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Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

Authors
  • Kubicz, Ewelina
  • Jasińska, Bożena
  • Zgardzińska, Bożena
  • Bednarski, Tomasz
  • Białas, Piotr
  • Czerwiński, Eryk
  • Gajos, Aleksander
  • Gorgol, Marek
  • Kamińska, Daria
  • Kapłon, Łukasz
  • Kochanowski, Andrzej
  • Korcyl, Grzegorz
  • Kowalski, Paweł
  • Kozik, Tomasz
  • Krzemień, Wojciech
  • Niedźwiecki, Szymon
  • Pałka, Marek
  • Raczyński, Lech
  • Rajfur, Zenon
  • Rudy, Zbigniew
  • And 9 more
Type
Published Article
Journal
Nukleonika
Publisher
De Gruyter Open Sp. z o.o.
Publication Date
Dec 01, 2015
Volume
60
Issue
4
Pages
749–753
Identifiers
DOI: 10.1515/nuka-2015-0135
Source
De Gruyter
Keywords
License
Green

Abstract

Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.

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