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Brain benzodiazepine receptors in fathead minnows and the behavioral response to alarm pheromone.

Authors
  • Rehnberg, B G1
  • Bates, E H
  • Smith, R J
  • Sloley, B D
  • Richardson, J S
  • 1 Department of Biology, University of Saskatchewan, Saskatoon, Canada. , (Canada)
Type
Published Article
Journal
Pharmacology Biochemistry and Behavior
Publisher
Elsevier
Publication Date
Jun 01, 1989
Volume
33
Issue
2
Pages
435–442
Identifiers
PMID: 2554343
Source
Medline
License
Unknown

Abstract

Fathead minnows (Pimephales promelas) exposed to 1 or 10 mg/l chlordiazepoxide showed normal alarm behavior during the presentation of alarm pheromone. Fish exposed to 20 mg/l drug, however, showed little or no behavioral alarm and did not appear sedated. A food extract stimulus presented after alarm pheromone led to a large foraging response by fish exposed to 20 mg/l chlordiazepoxide. Control fish or fish exposed to 1 to 10 mg/l drug showed less tendency to begin foraging. Exposure to 1, 10, or 20 mg/l chlordiazepoxide for 3 hr did not affect whole-brain concentrations of tryptophan, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, tyrosine, or dopamine. The binding of [3H]diazepam to specific brain receptor sites (KD = 10 nM, estimated Bmax = 3.3 fmol/mg wet weight) could be displaced by chlordiazepoxide (IC50 = 1.6 microM). These results suggest that benzodiazepine receptors in the central nervous system of lower vertebrates may function in ways similar to those in mammals, i.e., in the modulation of behavior in anxiety-like states.

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