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Bovine viral diarrhea virus-specific neutralizing antibodies induced by anti-idiotypic antibodies.

Authors
  • Onisk, D V
  • Donis, R
  • Kelling, C L
  • Srikumaran, S
Type
Published Article
Journal
Viral immunology
Publication Date
Jan 01, 1992
Volume
5
Issue
4
Pages
257–263
Identifiers
PMID: 1335258
Source
Medline
License
Unknown

Abstract

Two murine neutralizing monoclonal antibodies (MAbs), 4D8 and 6D11, recognizing epitopes on gp53, a surface glycoprotein of bovine viral diarrhea virus (BVDV), were used to generate anti-idiotypic antibodies (anti-ids) in a calf. The polyclonal anti-ids were isolated from serum by affinity chromatography on their respective Ab-1-Sepharose columns, followed by repeated adsorption on isotype-matched antibody-Sepharose columns. The anti-ids reacted specifically with their respective Ab-1, but not with isotype-matched controls. They also inhibited the binding of their Ab-1 to BVDV in a concentration-dependent manner. Mice immunized with the two anti-id preparations developed antibodies to BVDV, which neutralized the virus in vitro.

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