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Boosting immune response to hepatitis B DNA vaccine by coadministration of Prothymosin alpha-expressing plasmid.

Authors
  • Jin, Yanwen1
  • Cao, Cheng
  • Li, Ping
  • Liu, Xuan
  • Huang, Wei
  • Li, Chufang
  • Ma, Qingjun
Type
Published Article
Journal
Clinical and Diagnostic Laboratory Immunology
Publisher
American Society for Microbiology
Publication Date
Dec 01, 2005
Volume
12
Issue
12
Pages
1364–1369
Identifiers
PMID: 16339058
Source
Medline
Language
English
License
Unknown

Abstract

DNA vaccines induce protective humoral and cell-mediated immune responses in several animal models. However, compared to conventional vaccines, DNA vaccines usually induce poor antibody responses. In this study, we report that coadministration of a hepatitis B virus (HBV) DNA vaccine with prothymosin alpha as an adjuvant improves antibody responses to HBV S antigen. We also observed higher seroconversion rates and higher antibody titers. Prothymosin alpha appears to increase the number and affinity of hepatitis B surface antigen-specific, gamma interferon-secreting T cells and to enhance cellular immune response to the PreS2S DNA vaccine. Interestingly, administering the DNA separately from the prothymosin alpha plasmid abrogated the enhancement of DNA vaccine potency. The results suggest that prothymosin alpha may be a promising adjuvant for DNA vaccines.

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