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Bone marrow-derived mesenchymal stem cells improve rat islet graft revascularization by upregulating ISL1.

Authors
  • Wang, Ying1
  • Wang, Jing-Wen1
  • Li, Yang1
  • Tian, Xiao-Hui1
  • Feng, Xin-Shun1
  • Zhang, Shu-Cong1
  • Liu, Pei-Jun2
  • Xue, Wu-Jun1
  • Zheng, Jin1
  • Ding, Xiao-Ming1
  • 1 Department of Renal Transplantation, Hospital of Nephrology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic of China. , (China)
  • 2 Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, People's Republic of China. , (China)
Type
Published Article
Journal
The International Journal Of Cell Cloning
Publisher
Wiley (John Wiley & Sons)
Publication Date
Aug 01, 2021
Volume
39
Issue
8
Pages
1033–1048
Identifiers
DOI: 10.1002/stem.3378
PMID: 33754392
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Revascularization of the islet transplant is a crucial step that defines the success rate of patient recovery. Bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to promote revascularization; however, the underlying cellular mechanism remains unclear. Moreover, our liquid chromatography-tandem mass spectrometry results showed that BMSCs could promote the expression of insulin gene enhancer binding protein-1 (ISL1) in islets. ISL1 is involved in islets proliferation and plays a potential regulatory role in the revascularization of islets. This study identifies the ISL1 protein as a potential modulator in BMSCs-mediated revascularization of islet grafts. We demonstrated that the survival rate and insulin secretion of islets were increased in the presence of BMSCs, indicating that BMSCs promote islet revascularization in a coculture system and rat diabetes model. Interestingly, we also observed that the presence of BMSCs led to an increase in ISL1 and vascular endothelial growth factor A (VEGFA) expression in both islets and the INS-1 rat insulinoma cell line. In silico protein structure modeling indicated that ISL1 is a transcription factor that has four binding sites with VEGFA mRNA. Further results showed that overexpression of ISL1 increased both the abundance of VEGFA transcripts and protein accumulation, while inhibition of ISL1 decreased the abundance of VEGFA. Using a ChIP-qPCR assay, we demonstrated that direct molecular interactions between ISL1 and VEGFA occur in INS-1 cells. Together, these findings reveal that BMSCs promote the expression of ISL1 in islets and lead to an increase in VEGFA in islet grafts. Hence, ISL1 is a potential target to induce early revascularization in islet transplantation. ©AlphaMed Press 2021.

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