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A Bodian method for mounted frozen sections.

Authors
  • Herr, B E
  • Coleman, P D
  • Griggs, R C
Type
Published Article
Journal
Stain technology
Publication Date
Sep 01, 1976
Volume
51
Issue
5
Pages
261–265
Identifiers
PMID: 63161
Source
Medline
Language
English
License
Unknown

Abstract

For application of the Bodian method to frozen sections, cut frozen peripheral nerve or muscle at 10 mum and mount. Fix for 4 days in 18 parts 80% ethanol, 1 part 10% formalin, and 1 part glacial acetic acid. Fix central nervous tissue in the same mixture prior to freezing and sectioning, and after mounting postfix for 4 days. Impregnate by the Bodian procedure. The results equal Bodian stains of paraffin sections. The technique is simple and reliable. The use of 10 mum frozen sections produces little artifact and allows alternate serial sections to be stained with other techniques.

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