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The blue-green alga agmenellum quadruplicatum contains covalently closed DNA circles.

Authors
  • Roberts, T M
  • Koths, K E
Type
Published Article
Journal
Cell
Publisher
Elsevier
Publication Date
Dec 01, 1976
Volume
9
Issue
4 Pt 1
Pages
551–557
Identifiers
PMID: 827332
Source
Medline
License
Unknown

Abstract

This paper reports the first direct visualization of covalently closed, circular DNA from a blue-green alga. Agmenellum quadruplicatum lysates, fractionated on CsCl-ethidium bromide density gradients, yield a minor band of covalently closed DNA circles which we have analyzed by electron microscopy and agarose gel electrophoresis. The DNA consists of a number of a discrete classes of DNA circles ranging in molecular weight from 3 X 10(6) daltons to 65-80 X 10(6) daltons. The function of these circles is at present entirely unknown.

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