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Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves.

Authors
  • Santana, André M1
  • Silva, Daniela G2
  • Thomas, Funmilola C3
  • Bernardes, Priscila A2
  • Pizauro, Lucas J L2
  • Santana, Clarissa H2
  • Burchmore, Richard J S4
  • Eckersall, Peter D5
  • Fagliari, José J2
  • 1 Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal, SP, Brazil. Electronic address: [email protected] , (Brazil)
  • 2 Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, São Paulo State University (FCAV/UNESP), Jaboticabal, SP, Brazil. , (Brazil)
  • 3 Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, Nigeria. , (Niger)
  • 4 Institute of Infection, Immunity and Inflammation, Glasgow Polyomics Facility, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom. , (United Kingdom)
  • 5 Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom. , (United Kingdom)
Type
Published Article
Journal
Veterinary immunology and immunopathology
Publication Date
Sep 01, 2018
Volume
203
Pages
30–39
Identifiers
DOI: 10.1016/j.vetimm.2018.07.014
PMID: 30243370
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The study aimed to evaluate clinical signs, blood serum acute phase proteins (APP) and iron dynamics during the acute phase response (APR) of Salmonella Dublin experimentally infected Murrah buffalo calves. Six buffalo calves constituted the control group (CNT) and six were orally inoculate with 108 CFU of S. Dublin (INF). Clinical evaluation was performed, rectal swabs to detect S. Dublin strains were collected and venous blood was sampled before and throughout seven days after inoculation. The APP fractions β-haptoglobin, α-haptoglobin, ceruloplasmin and transferrin were analyzed by 1-D and 2-D electrophoresis. Proteins were identified using LC/ESI-MS/MS and NCBI database. Plasma fibrinogen, serum iron and serum haptoglobin concentrations were measured. The inoculation of 108 CFU of S. Dublin was effective in inducing clinical signs of Salmonellosis, such as hyperthermia and diarrhea. 1-DE showed that β and α-haptoglobin increased 204% (p = 0.008) and 184% (p = 0.022) 48 h after inoculation (HAI), respectively, with highest concentrations 120 HAI (498% increased, p = 0.012; 431% increased, p = 0.011) and 168 HAI (492% increased, p = 0.019; 523% increased, p = 0.028). 2-DE showed that the expression of two spots, identified as β-haptoglobin, were increased 693% (p = 0.0006) and 580% (p = 0.0003) 168 HAI, respectively, while one spot, identified as α-haptoglobin, increased 714% (p = 0.040). Haptoglobin concentrations increased 1339% (p < 0.0001) 168 HAI. 1-DE showed that ceruloplasmin increased 42% (p = 0.034) 48 HAI, with highest concentration 120 HAI (133% increased, p = 0.022). 2-DE showed that the expression of two spots, identified as ceruloplasmin, were increased 218% (p = 0.0153) and 85% (p = 0.0143) 168 HAI, respectively. Fibrinogen increased 78% (p = 0.012) 96 HAI, with highest concentration 120 HAI (increased 114%, p = 0.002). Iron decreased 33% 24 HAI (p = 0.015) and 37% 72 HAI (p = 0.029), and began to be restored 96 HAI. 1-DE showed that transferrin decreased 23% 120 HAI (p = 0.047), and that values were restored 168 HAI. 2-DE showed that expression patterns of transferrin comparing 0 h and 168 HAI were similar, evidencing that values were restored 168 HAI. In conclusion, the inoculation of 108 CFU was effective in inducing hyperthermia and diahrrea. β and α-haptoglobin, ceruloplasmin and fibrinogen worked as positive APP during the APR to S. Dublin infection and are potential biomarker candidates. Concentrations of iron and transferrin decreased during the infection, highlighting the fact that mechanisms for restricting iron availability are part of the APR triggered against S. Dublin infection in buffalo calves. Copyright © 2018 Elsevier B.V. All rights reserved.

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