Affordable Access

deepdyve-link
Publisher Website

Blood culture-PCR to optimise typhoid fever diagnosis after controlled human infection identifies frequent asymptomatic cases and evidence of primary bacteraemia.

Authors
  • Darton, Thomas C1
  • Zhou, Liqing2
  • Blohmke, Christoph J2
  • Jones, Claire2
  • Waddington, Claire S2
  • Baker, Stephen3
  • Pollard, Andrew J2
  • 1 Oxford Vaccine Group, Department of Paediatrics, NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, United Kingdom; The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam. Electronic address: [email protected]
  • 2 Oxford Vaccine Group, Department of Paediatrics, NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, United Kingdom.
  • 3 The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, Oxford University, Oxford, United Kingdom.
Type
Published Article
Journal
The Journal of infection
Publication Date
Jan 24, 2017
Identifiers
DOI: 10.1016/j.jinf.2017.01.006
PMID: 28130144
Source
Medline
Keywords
License
Unknown

Abstract

Overall the culture-PCR assay performed well, identifying extra typhoid cases compared with routine blood culture alone. Despite limitations to widespread field-use, the benefits of increased diagnostic yield, reduced blood volume and faster turn-around-time, suggest that this assay could enhance laboratory typhoid diagnostics in research applications and high-incidence settings.

Report this publication

Statistics

Seen <100 times