Affordable Access

deepdyve-link
Publisher Website

A blood capillary plexus-derived population of progenitor cells contributes to genesis of the dermal lymphatic vasculature during embryonic development.

Authors
  • Pichol-Thievend, Cathy1
  • Betterman, Kelly L2
  • Liu, Xiaolei3
  • Ma, Wanshu3
  • Skoczylas, Renae1
  • Lesieur, Emmanuelle1
  • Bos, Frank L4
  • Schulte, Dorte5
  • Schulte-Merker, Stefan5
  • Hogan, Benjamin M1
  • Oliver, Guillermo3
  • Harvey, Natasha L6
  • Francois, Mathias7
  • 1 Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia. , (Australia)
  • 2 Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide 5001, South Australia, Australia. , (Australia)
  • 3 Center for Vascular and Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL 60611, USA.
  • 4 Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Centre, Utrecht 3584CT, The Netherlands. , (Netherlands)
  • 5 University of Münster, 48149 Münster, Germany Institute for Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, Westfälische Wilhelms-Universität Münster (WWU), Mendelstrasse 7, 48149 Münster and CiM Cluster of Excellence, Germany. , (Germany)
  • 6 Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide 5001, South Australia, Australia [email protected] [email protected] , (Australia)
  • 7 Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia [email protected] [email protected] , (Australia)
Type
Published Article
Journal
Development
Publisher
The Company of Biologists
Publication Date
May 17, 2018
Volume
145
Issue
10
Identifiers
DOI: 10.1242/dev.160184
PMID: 29773646
Source
Medline
Keywords
License
Unknown

Abstract

Despite the essential role of the lymphatic vasculature in tissue homeostasis and disease, knowledge of the organ-specific origins of lymphatic endothelial progenitor cells remains limited. The assumption that most murine embryonic lymphatic endothelial cells (LECs) are venous derived has recently been challenged. Here, we show that the embryonic dermal blood capillary plexus constitutes an additional, local source of LECs that contributes to the formation of the dermal lymphatic vascular network. We describe a novel mechanism whereby rare PROX1-positive endothelial cells exit the capillary plexus in a Ccbe1-dependent manner to establish discrete LEC clusters. As development proceeds, these clusters expand and further contribute to the growing lymphatic system. Lineage tracing and analyses of Gata2-deficient mice confirmed that these clusters are endothelial in origin. Furthermore, ectopic expression of Vegfc in the vasculature increased the number of PROX1-positive progenitors within the capillary bed. Our work reveals a novel source of lymphatic endothelial progenitors employed during construction of the dermal lymphatic vasculature and demonstrates that the blood vasculature is likely to remain an ongoing source of LECs during organogenesis, raising the question of whether a similar mechanism operates during pathological lymphangiogenesis.

Report this publication

Statistics

Seen <100 times