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Blastogenic response of lymphocytes separated from bone marrow to allogeneic lymphoid cells in vitro

Authors
  • Y. Yoshida
  • D. G. Osmond
Publication Date
Nov 01, 1971
Source
PMC
Keywords
License
Unknown

Abstract

Fractions of cells were separated from the bone marrow and spleen of Lewis rats by brief centrifugation in linear sucrose-serum density gradients and were cultured in vitro either alone or mixed with F1 (Lewis × Brown Norway) hybrid rat lymphoid cells. After 4 days the incorporation of [3H]thymidine into DNA was measured by scintillation counting, and the morphology and incidence of [3H]thymidine-labelled cells were determined in radioautographs.

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