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Bivalency in Drosophila embryos is associated with strong inducibility of Polycomb target genes.

Authors
  • Akmammedov, Arslan1
  • Geigges, Marco1
  • Paro, Renato1, 2
  • 1 Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. , (Switzerland)
  • 2 Faculty of Science, University of Basel, Basel, Switzerland. , (Switzerland)
Type
Published Article
Journal
Fly
Publisher
Landes Bioscience
Publication Date
Jan 01, 2019
Volume
13
Issue
1-4
Pages
42–50
Identifiers
DOI: 10.1080/19336934.2019.1619438
PMID: 31094269
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Polycomb group (PcG) and Trithorax group (TrxG) proteins orchestrate development of a multicellular organism by faithfully maintaining cell fate decisions made early in embryogenesis. An important chromatin mark connected to PcG/TrxG regulation is bivalent domains, the simultaneous presence of H3K27me3 and H3K4me3 on a given locus, originally identified in mammalian embryonic stem cells but considered to be absent in invertebrates. Here, we provide evidence for the existence of bivalency in fly embryos. Using a recently described PcG reporter fly line, we observed a strong reporter inducibility in the embryo and its sharp decrease in larval and adult stages. Analysis of the chromatin landscape of the reporter revealed a strong signal for the repressive PcG mark, H3K27me3, in all three developmental stages and, surprisingly, a strong signal for a transcriptionally activating H3K4me3 mark in the embryo. Using re-chromatin immunoprecipitation experiments, bivalent domains were also uncovered at endogenous PcG targets like the Hox genes.

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