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BisQC: an operational pipeline for multiplexed bisulfite sequencing.

Authors
  • Chen, Gary G
  • Diallo, Alpha B
  • Poujol, Raphaël
  • Nagy, Corina
  • Staffa, Alfredo
  • Vaillancourt, Kathryn
  • Lutz, Pierre-Eric
  • Ota, Vanessa K
  • Mash, Deborah C
  • Turecki, Gustavo
  • Ernst, Carl
Type
Published Article
Journal
BMC Genomics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 01, 2014
Volume
15
Pages
290–290
Identifiers
DOI: 10.1186/1471-2164-15-290
PMID: 24734894
Source
Medline
License
Unknown

Abstract

We demonstrate several quality control checkpoints at the library preparation, pre-sequencing, post-sequencing, and post-alignment stages, which should prove useful in determining sample and processing quality. We also determine that including a significant portion of non-bisulfite converted DNA with bisulfite converted DNA has a minimal impact on usable bisulfite read output.

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