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BisQC: an operational pipeline for multiplexed bisulfite sequencing.

Authors
Type
Published Article
Journal
BMC Genomics
1471-2164
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
15
Pages
290–290
Identifiers
DOI: 10.1186/1471-2164-15-290
PMID: 24734894
Source
Medline
License
Unknown

Abstract

We demonstrate several quality control checkpoints at the library preparation, pre-sequencing, post-sequencing, and post-alignment stages, which should prove useful in determining sample and processing quality. We also determine that including a significant portion of non-bisulfite converted DNA with bisulfite converted DNA has a minimal impact on usable bisulfite read output.

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