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Biotransformation of homofolate from a growth inhibitor to a growth-promoting derivative.

Authors
Type
Published Article
Journal
Chemotherapy
Publication Date
Volume
29
Issue
6
Pages
436–441
Identifiers
PMID: 6418446
Source
Medline
License
Unknown

Abstract

Homofolic acid (HF) was found to inhibit the growth of Lactobacillus casei ATCC 7469 in 100 ng/ml or higher concentrations in the presence of folic acid (PGA) on short (24-48 h) incubation. HF blocked the uptake of 3HPGA into L. casei cell suspensions. On prolonged (3-5 days) incubation HF (greater than 10 ng/ml) promoted growth of L. casei both in the presence and absence of PGA. No growth was obtained when both PGA and HF were omitted. Thus, HF was apparently biotransformed from an inhibitory analog into a growth-promoting HF derivative. Indeed, sterile filtrates from HF-grown cultures supported growth of L. casei in a folate-free medium on short incubation, supporting the notion that a folate-like active compound was formed from HF on prolonged incubation with L. casei. In the presence of homopteorate (10-60 micrograms/ml) no transformation of HF into folate-active compounds took place.

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