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BIOORTHOGONALLY APPLICABLE FLUOROGENIC CYANINE-TETRAZINES FOR NO-WASH SUPER-RESOLUTION IMAGING.

Authors
  • Knorr, Gergely
  • Kozma, Eszter
  • Schaart, Judith
  • Németh, Krisztina
  • Török, György
  • Kele, Peter
Type
Published Article
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society
Publication Date
Feb 12, 2018
Identifiers
DOI: 10.1021/acs.bioconjchem.8b00061
PMID: 29431990
Source
Medline
License
Unknown

Abstract

The synthesis, fluorogenic characterization and labeling application of four tetrazine-quenched cyanine probes with emission maxima in the red - far red range is reported. Fluorescence of the cyanine-cores is quenched via through-bond-energy-transfer (TBET) exerted by a bioorthogonal tetrazine unit. Upon bioorthogonal labeling reaction with cyclooctyne tagged proteins, the quenching effect ceases, thus the fluorescence reinstates, resulting in an increase in fluorescence intensity. As a rare example amongst indocyanines, one of our new probes was found suitable in STED-based super-resolution imaging. The applicability of this fluorogenic Tet-Cy3 probe was therefore further demonstrated in the bioorthogonal labeling of cytoskeletal protein, actin, with subsequent super-resolution microscopy (STED) imaging even under no-wash conditions.

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