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Biomimetic Hydrogels Loaded with Nanofibers Mediate Sustained Release of pDNA and Promote In Situ Bone Regeneration.

Authors
  • Huang, Lin1
  • Zhang, Zhijie2
  • Guo, Mingtao3
  • Pan, Cile4
  • Huang, Zhiguan1
  • Jin, Junfei2
  • Li, Yuhe1
  • Hou, Xiaohui1
  • Li, Wenqiang1
  • 1 Engineering Technology Research Center for Sports Assistive Devices of Guangdong, Guangzhou Sport University, Guangzhou, 510630, China. , (China)
  • 2 Luoyang Orthopedic Hospital of Henan Province, Luoyang, 641007, China. , (China)
  • 3 Sports Department, Shenzhen Polytechnic, Shenzhen, 518036, China. , (China)
  • 4 Department of Materials Science and Engineering, Jinan University, Guangzhou, 510632, China. , (China)
Type
Published Article
Journal
Macromolecular bioscience
Publication Date
Apr 01, 2021
Volume
21
Issue
4
Identifiers
DOI: 10.1002/mabi.202000393
PMID: 33625790
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Polymer hydrogels are generally insufficient biomechanics, strong resistance to cell adhesion, and weak bioactivity which limits their application in bone tissue engineering considerably. In order to develop a bone tissue engineering material with both good mechanical properties, osteogenic and angiogenic activity. Nanofibers carrying DNA plasmid (pNF) are introduced to gelatin methacryloyl (GelMA) and thiolated chitosan (TCS) system for preparing a novel GelMA/TCS/pNF composite hydrogel with dual network structure. By characterization of the compressive measurements, the resulting composite scaffold shows greatly enhanced mechanical strength (0.53 MPa) and is not damaged after 20 cycles of compression. And the fabricated composite scaffold displays sustained release of bone morphogenetic protein-2 that can induce osteogenic differentiation and angiopoietin-1 that promotes vascularization. The cell experiment shows that this system can significantly promote MC3T3-E1 cell attachment, proliferation, as well as osteogenic-related and angiogenic-related genes expression of MC3T3-E1 cells. Moreover, the in vivo results show that the composite scaffold with activated gene fibers can significantly promote osteogenesis and vascularization leading to favorable capacity of bone regeneration, meaning that the resulting biomimetic composite hydrogel scaffolds are excellent candidates for bone repair materials. © 2021 Wiley-VCH GmbH.

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