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Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function

Authors
  • Mi, Zhanhu1
  • Dong, Yanyun2
  • Wang, Zhibiao3
  • Ye, Peng1
  • 1 General Hospital of Ningxia Medical University, 804 South Shengli Street, Yinchuan, Ningxia, 750004, People’s Republic of China , Yinchuan (China)
  • 2 General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, People’s Republic of China , Yinchuan (China)
  • 3 Rizhao Central Hospital, Rizhao, Shandong, 276000, People’s Republic of China , Rizhao (China)
Type
Published Article
Journal
Journal of Orthopaedic Surgery and Research
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jul 28, 2021
Volume
16
Issue
1
Identifiers
DOI: 10.1186/s13018-021-02611-2
Source
Springer Nature
Keywords
Disciplines
  • Research Article
License
Green

Abstract

BackgroundOsteosarcoma (OS) is a type of bone cancer that occurs in children and adolescents at a rate of 5%. The purpose of this study is to explore the lncRNA GNAS-AS1 expression profile, prognosis significance in OS, and biological effect on OS cell function.MethodsOne hundred eight pairs of tissues were collected, and OS cell lines were purchased. lncRNA GNAS-AS1 expression in these tissues and cells were analyzed by qRT-PCR. Clinical data were analyzed using chi-square tests, Kaplan-Meier curves (log-rank test), and Cox regression. CCK-8 and transwell assay were conducted to analyze the effect of lncRNA GNAS-AS1 on cell proliferation, invasion, and migration. The downstream miRNA was presumed.ResultsThe expression of lncRNA GNAS-AS1 was significantly increased in OS cells and tissues, and related to Enneking staging and distant metastasis. Patients with high lncRNA GNAS-AS1 expression represented shorter overall survival and was an independent prognostic predictor of OS. LncRNA GNAS-AS1 knockdown inhibited cell proliferation, migration, and invasion by regulated miR-490-3p partly at least.ConclusionsLncRNA GNAS-AS1 can be used as a prognostic indicator and its inhibition suppress the development of OS, suggesting its value as novel therapeutic strategies in OS.

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