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Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms.

Authors
  • Frank, L
  • Markova, S
  • Remmel, N
  • Vysotski, E
  • Gitelson, I
Type
Published Article
Journal
Luminescence : the journal of biological and chemical luminescence
Publication Date
Jan 01, 2007
Volume
22
Issue
3
Pages
215–220
Identifiers
PMID: 17286244
Source
Medline
License
Unknown

Abstract

The Ca(2+)-regulated photoprotein obelin has been examined as a label for bioluminescence immunoassay of infective agents. The hepatitis B virus (HbsAg) and the bacteria Escherichia coli and Shigella sonnei lipopolysaccharide (LPS) were chosen as model antigens. Chemically synthesized obelin-corresponding antibody conjugates were used in a solid-phase microplate immunoassay. The sensitivities achieved by the assay were 0.25 ng/mL for S. sonnei LPS and 0.375 ng/mL for HbsAg. A novel, filter-based immunoassay to determine bacterial admixtures in the environment was proposed. The NanoCeram filters were effectively applied to 'trap' and pre-concentrate pathogens from samples under study for the purposes of further detection and measurement of the absorbed material by bioluminescence immunoassay.

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