Affordable Access

deepdyve-link
Publisher Website

Bioluminescence reporter gene imaging of human embryonic stem cell survival, proliferation, and fate.

Authors
  • Wilson, Kitchener D
  • Huang, Mei
  • Wu, Joseph C
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2009
Volume
574
Pages
87–103
Identifiers
DOI: 10.1007/978-1-60327-321-3_8
PMID: 19685302
Source
Medline
License
Unknown

Abstract

The discovery of human embryonic stem cells (hESCs) has dramatically increased the tools available to medical scientists interested in regenerative medicine. However, direct injection of hESCs, and cells differentiated from hESCs, into living organisms has thus far been hampered by significant cell death, teratoma formation, and host immune rejection. Understanding the in vivo hESC behavior after transplantation requires novel imaging techniques to longitudinally monitor hESC localization, proliferation, and viability. Molecular imaging, and specifically bioluminescent reporter gene imaging, has given investigators a high-throughput, inexpensive, and sensitive means for tracking in vivo cell proliferation over days, weeks, and even months. This advancement has significantly increased the understanding of the spatiotemporal kinetics of hESC engraftment and proliferation in living subjects. In this chapter, the specific materials and methods needed for tracking stem cell proliferation with bioluminescence imaging will be described.

Report this publication

Statistics

Seen <100 times