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Biolistics-based gene silencing in plants using a modified particle inflow gun.

Authors
  • Davies, Kevin M
  • Deroles, Simon C
  • Boase, Murray R
  • Hunter, Don A
  • Schwinn, Kathy E
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2013
Volume
940
Pages
63–74
Identifiers
DOI: 10.1007/978-1-62703-110-3_6
PMID: 23104334
Source
Medline
License
Unknown

Abstract

RNA interference (RNAi) is one of the most commonly used techniques for examining the function of genes of interest. In this chapter we present two examples of RNAi that use the particle inflow gun for delivery of the DNA constructs. In one example transient RNAi is used to show the function of an anthocyanin regulatory gene in flower petals. In the second example stably transformed cell cultures are produced with an RNAi construct that results in a change in the anthocyanin hydroxylation pattern.

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