Affordable Access

deepdyve-link
Publisher Website

Thymosin Beta-4 and Ciprofloxacin Adjunctive Therapy Improves Pseudomonas aeruginosa-Induced Keratitis.

Authors
  • Carion, Thomas W1
  • Ebrahim, Abdul Shukkur2
  • Kracht, David3
  • Agrawal, Aditya4
  • Strand, Eliisa5
  • Kaddurah, Omar6
  • McWhirter, Cody R7
  • Sosne, Gabriel8
  • Berger, Elizabeth A9
  • 1 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 2 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 3 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 4 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 5 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 6 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 7 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 8 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
  • 9 Department of Ophthalmology, Visual & Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USA. [email protected]
Type
Published Article
Journal
Cells
Publisher
MDPI AG
Publication Date
Sep 20, 2018
Volume
7
Issue
10
Identifiers
DOI: 10.3390/cells7100145
PMID: 30241380
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

With increasing multidrug resistance and contraindication for corticosteroid use, the goal of this study was to develop thymosin beta-4 (Tβ4) as an adjunctive therapy to antibiotics for the treatment of bacterial keratitis that effectively promotes enhanced wound healing, host defense, and inflammation resolution. Disease outcome was assessed by clinical score, slit lamp photography, and histopathology. Cytokine profile, bacterial load, PMN infiltration, and Griess and reactive oxygen species (ROS) levels were determined. Adjunct Tβ4 treatment resulted in a significant improvement compared to PBS, Tβ4, and most remarkably, ciprofloxacin, correlating with changes in mediators of inflammation and wound healing. Collectively, these data provide evidence that wound healing is an essential aspect in the development of new therapies to treat corneal infection. Use of adjunctive Tβ4 provides a more efficacious approach for bacterial keratitis by addressing both the infectious pathogen and deleterious host response.

Report this publication

Statistics

Seen <100 times