Affordable Access

deepdyve-link
Publisher Website

Bioengineering and characterization of DNA-protein assemblies floating on supported membranes.

Authors
Type
Published Article
Journal
Biotechnology and Bioengineering
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 12, 2002
Volume
77
Issue
2
Pages
225–231
Identifiers
DOI: 10.1002/bit.10111
PMID: 11753930
Source
USPC - SET - SVS
License
White

Abstract

A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors.

Report this publication

Statistics

Seen <100 times