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Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts

Authors
  • Sekar, Sudharshan1
  • Mahadevan, Surianarayanan1
  • Vijayaraghavan, Ranganathan2
  • Mandal, Asit Baran1
  • MacFarlane, D. R.2
  • 1 Central Leather Research Institute (CLRI), Thermochemical Lab, Chemical Engineering Department, Adyar, Chennai, Tamil Nadu, 600020, India , Chennai (India)
  • 2 Monash University, School of Chemistry, Clayton Campus, Melbourne, VIC, 3800, Australia , Melbourne (Australia)
Type
Published Article
Journal
Applied Microbiology and Biotechnology
Publisher
Springer-Verlag
Publication Date
Apr 18, 2012
Volume
97
Issue
4
Pages
1767–1774
Identifiers
DOI: 10.1007/s00253-012-4009-0
Source
Springer Nature
Keywords
License
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Abstract

Choline-based biocompatible salts were used as “nutrients” for the growth of Staphylococcus lentus bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power–time curve in an investigation monitored online by reaction calorimetry. From the power–time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of S. lentus were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (YQ/S) consumption and oxygen (YQ/O) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry.

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