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THE BIOCHEMICAL CONVERSION OF 2-HYDROXY-4-METHYLTHIOBUTYRIC ACID INTO METHIONINE BY THE RAT IN VITRO.

Authors
  • LANER, B W Jr
Type
Published Article
Journal
The Biochemical journal
Publication Date
Jun 01, 1965
Volume
95
Pages
683–687
Identifiers
PMID: 14342503
Source
Medline
Keywords
License
Unknown

Abstract

1. The conversion of 2-hydroxy-4-methylthiobarbituric acid into methionine requires a flavine coenzyme, which can be partially replaced by NAD or NADP, and glutamine, which can be partially replaced by asparagine. The system also requires aerobic conditions. 2. There does not appear to be a requirement for a metal ion: Mn(2+) is slightly inhibitory and Cu(2+) is strongly inhibitory. 3. Of the kidney, liver, brain, small intestine and muscle, the kidney possesses the greatest conversion activity/g. of tissue, but on a total organ basis the liver is the more important organ. Within the liver cells most of the activity is present in the supernatant fraction.

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