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Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58.

Authors
  • Hasan, Khomaini
  • Fortova, Andrea
  • Koudelakova, Tana
  • Chaloupkova, Radka
  • Ishitsuka, Mayuko
  • Nagata, Yuji
  • Damborsky, Jiri
  • Prokop, Zbynek
Type
Published Article
Journal
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Publication Date
Mar 01, 2011
Volume
77
Issue
5
Pages
1881–1884
Identifiers
DOI: 10.1128/AEM.02109-10
PMID: 21193677
Source
Medline
License
Unknown

Abstract

We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

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