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Biochemical aspects of the soybean response to herbivory injury by the brown stink bug Euschistus heros (Hemiptera: Pentatomidae).

Authors
  • Timbó, Renata Velozo1
  • Hermes-Lima, Marcelo2
  • Silva, Luciano Paulino3
  • Mehta, Angela3
  • Moraes, Maria Carolina Blassioli3
  • Paula, Débora Pires3
  • 1 Department of Cell Biology, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, Brazil; Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil. , (Brazil)
  • 2 Department of Cell Biology, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal, Brazil. , (Brazil)
  • 3 Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil. , (Brazil)
Type
Published Article
Journal
PLoS ONE
Publisher
Public Library of Science
Publication Date
Jan 01, 2014
Volume
9
Issue
10
Identifiers
DOI: 10.1371/journal.pone.0109735
PMID: 25333272
Source
Medline
License
Unknown

Abstract

Plant defense response is an elaborate biochemical process shown to depend on the plant genetic background and on the biological stressor. This work evaluated the soybean biochemical foliar response to brown stink bug herbivory injury through an analysis of redox metabolism and proteomic 2DE profiles of susceptible (BRS Silvania RR) and resistant (IAC-100) varieties. The activity of lipoxygenase-3, guaiacol peroxidase, catalase and ascorbate peroxidase was monitored every 24 h up to 96 h. In the susceptible variety, injury caused an increase in the activities of lipoxygenase 3 and guaiacol peroxidase, no change in ascorbate peroxidase, and a decrease in catalase. In the resistant variety, injury did not cause an alteration of any of these enzymes. The proteomic profiles were evaluated after 24 h of injury and revealed to have a similar proportion (4-5%) of differential protein expression in both varieties. The differential proteins, identified by mass spectrometry, in the susceptible variety were related to general stress responses, to plant defense, and to fungal infections. However, in the resistant variety, the identified change in protein profile was related to Calvin cycle enzymes. While the susceptible variety showed adaptive changes in redox metabolism and expression of stress-responsive proteins, the resistant showed a defense response to circumvent the biological stressor.

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