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Binding interaction of gamma aminobutyric acid A and B receptors in cell culture.

Authors
Type
Published Article
Journal
Neuroreport
0959-4965
Publisher
Ovid Technologies (Wolters Kluwer) - Lippincott Williams & Wilkins
Publication Date
Volume
2
Issue
9
Pages
541–543
Identifiers
PMID: 1661194
Source
Medline
License
Unknown

Abstract

We have examined the effects of 0.1 mM baclofen and pertussis toxin treatment on gamma aminobutyric acid A (GABAA) receptor binding in whole cells and in membrane of cells from cerebellar primary culture. Baclofen, a GABAB receptor ligand, caused a marked decrease in the bicuculline sensitive (GABAA) binding of [3H]muscimol on the whole cells, but failed to inhibit specific [3H]muscimol binding to cells pretreated with pertussis toxin. The effect of baclofen was only seen in whole cells and not in cell membranes. These findings suggest that activation of GABAB receptor reduces GABAA binding sites through a GTP binding protein.

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