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Binding of functionalized paramagnetic nanoparticles to bacterial lipopolysaccharides and DNA.

Authors
  • Bromberg, Lev
  • Chang, Emily P
  • Alvarez-Lorenzo, Carmen
  • Magariños, Beatriz
  • Concheiro, Angel
  • Hatton, T Alan
Type
Published Article
Journal
Langmuir
Publisher
American Chemical Society
Publication Date
Jun 01, 2010
Volume
26
Issue
11
Pages
8829–8835
Identifiers
DOI: 10.1021/la904589p
PMID: 20155986
Source
Medline
License
Unknown

Abstract

Magnetite and metallic cobalt-based nanoparticles with sizes ranging from 10 to 300 nm and surface-functionalized with poly(hexamethylene biguanide) (PHMBG) are introduced as capable lipopolysaccharide (LPS)-sequestering agents. The nanoparticles efficiently bind to whole E. coli cells and can be used to separate the cells effectively from suspension using a magnet. A fluorescence dye displacement assay shows strong affinities of the nanoparticles for lipid A, the glycolipid component of LPS responsible for septic shock. The particle-lipid A affinity is of the same order of magnitude or higher than that of polymyxin B. The affinity of smaller (< 50 nm) magnetite particles modified with PHMBG to lipid A is several-fold higher than that of their larger counterparts (> 100 nm) due to their higher surface area to volume ratio. The nanoparticles possess high saturation capacity for double-stranded lambdaDNA from E. coli, with which particle-polyelectrolyte complexes are formed. The PHMBG-modified nanoparticles are potent bactericides, inhibiting E. coli viability and growth at concentrations at < or = 10 microg/mL.

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