Affordable Access

Binding of calcium and magnesium by the contractile elements.

Authors
  • BOZLER, E
Type
Published Article
Journal
The Journal of general physiology
Publication Date
Jul 20, 1955
Volume
38
Issue
6
Pages
735–742
Identifiers
PMID: 13242760
Source
Medline
Keywords
License
Unknown

Abstract

Using a technique for determining Ca and Mg based on Schwarzenberg's method of titration with ethylenediamine tetraacetic acid (EDTA), it was found that glycerol-extracted muscle fibers contain on the average 0.58 millimole Ca and 0.55 millimole Mg per kg. muscle. The fibers take up additional Ca or Mg from dilute solutions of these metals, but in KCl solutions, the excess is exchanged for K ions. Inorganic pyrophosphate (PP) removes part of the bound Mg, no Ca; EDTA removes predominantly Ca, but never more than about one-half the total amount. These results are discussed in relation to previous observations on the effects of PP and EDTA on mechanical properties and contractility of extracted muscle fibers. After the partial loss of bound divalent metals, muscle fibers swell in dilute salt solutions; they also contract slightly and become more translucent.

Report this publication

Statistics

Seen <100 times