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Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods

Authors
Journal
Biosensors and Bioelectronics
0956-5663
Publisher
Elsevier
Publication Date
Volume
22
Issue
6
Identifiers
DOI: 10.1016/j.bios.2006.03.010
Keywords
  • T-2 Mycotoxin
  • Immune Assay
  • Spectroscopic Ellipsometry
  • Total Internal Reflection
  • Surface Plasmon Resonance (Spr)
  • Qcm Impedance Spectroscopy

Abstract

Abstract A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 μg/ml down to 0.15 ng/ml. Association constants of 1.4 × 10 6 and 1.9 × 10 7 mol −1 s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4–5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies.

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