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An antibody-specific method for the removal of an endopeptidase from the plant carboxypeptidase, phaseolain

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
41
Issue
3
Identifiers
DOI: 10.1016/0006-291x(70)90051-3
Disciplines
  • Biology

Abstract

Abstract Extensive fractionation by conventional procedures only allows partial purification of phaseolain, a carboxypeptidase-like enzyme from French beans. Such preparations still contain an endopeptidase contaminant (proteinase “c”). However, proteinase “c” can be purified by methods including ion-exchange chromatography, and insoluble antibody to proteinase “c” has been used to remove all traces of this endopeptidase from partially purified phaseolain preparations. Phaseolain now appears to be a true carboxypeptidase.

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