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Evidence that the C-terminus of OprM is involved in the assembly of the VceAB-OprM efflux pump

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
584
Issue
8
Identifiers
DOI: 10.1016/j.febslet.2010.02.066
Keywords
  • Involvement Of C-Terminus Of Oprm In Assembly Of Vceab-Oprm
Disciplines
  • Biology

Abstract

Abstract Although the architecture of tripartite multiple drug resistance (MDR) efflux pumps of Gram-negative bacteria has been well characterized, the means by which the components recognize each other and assemble into a functional pump remains obscure. In this study we present evidence that the C-terminal domain of the Pseudomonas aeruginosa OprM and the α-helical hairpin domain of Vibrio cholerae VceA play an important role in the recognition/specificity/recruitment step in the assembly of a functional, VceAB-OprM chimeric efflux pump. To our knowledge, this is the first evidence directly linking the C-terminal domain of an outer membrane efflux protein to its recruitment during the assembly of a tripartite efflux pump.

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