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Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution

Authors
  • Conceição, Natália1, 2
  • Rodrigues, Wellington Francisco3
  • de Oliveira, Kessys Lorrânya Peralta2
  • da Silva, Lucas Emanuel Pinheiro1
  • de Souza, Laís Rezende Cardoso1
  • da de Cunha Hueb Barata Oliveira, Cristi...4
  • de Oliveira, Adriana Gonçalves1, 3
  • 1 Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, 330, 38015-050, Uberaba, Minas Gerais, Brazil , Uberaba (Brazil)
  • 2 Instituto Federal de Educação, Ciência e Tecnologia de Rondônia, Colorado Do Oeste, Rondônia, Brazil , Colorado Do Oeste (Brazil)
  • 3 Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil , Uberaba (Brazil)
  • 4 Instituto de Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil , Uberaba (Brazil)
Type
Published Article
Journal
Annals of Clinical Microbiology and Antimicrobials
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Sep 17, 2020
Volume
19
Issue
1
Identifiers
DOI: 10.1186/s12941-020-00386-8
Source
Springer Nature
Keywords
License
Green

Abstract

This study aimed to evaluate the accuracy of disk diffusion and Etest methods, compared to that of the broth dilution reference method for identifying beta-lactam susceptibilities of Penicillin-Resistant, Ampicillin-Susceptible Enterococcus faecalis (PRASEF) isolates. Fifty-nine PRASEF and 15 Penicillin-Susceptible, Ampicillin-Susceptible E. faecalis (PSASEF) clinical nonrepetitive isolates were evaluated. The effectiveness of five beta-lactams (ampicillin, amoxicillin, imipenem, penicillin, and piperacillin) was tested. All antimicrobial susceptibility tests were performed and interpreted according to the Clinical and Laboratory Standards Institute guidelines. Interpretative discrepancies, such as essential agreement, categorical agreement, and errors, were assessed. The acceptability was ≥ 90% for both categorical agreement and essential agreement. Etest proved to be an accurate method for testing beta-lactam susceptibilities of the emerging PRASEF isolates, disk diffusion presented poor performance, particularly for imipenem and piperacillin.

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