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A benzimidazole-based ruthenium(IV) complex inhibits Pseudomonas aeruginosa biofilm formation by interacting with siderophores and the cell envelope, and inducing oxidative stress.

Authors
  • Czerwonka, Grzegorz1
  • Gmiter, Dawid1
  • Guzy, Anna1
  • Rogala, Patrycja2
  • Jabłońska-Wawrzycka, Agnieszka2
  • Borkowski, Andrzej3
  • Cłapa, Tomasz4
  • Narożna, Dorota4
  • Kowalczyk, Paweł5
  • Syczewski, Marcin6
  • Drabik, Marcin7
  • Dańczuk, Magdalena8
  • Kaca, Wiesław1
  • 1 a Department of Microbiology, Institute of Biology , Jan Kochanowski University in Kielce , Poland. , (Poland)
  • 2 b Institute of Chemistry , Jan Kochanowski University in Kielce , Poland. , (Poland)
  • 3 c Faculty of Geology, Geomicrobiology Laboratory , University of Warsaw , Warsaw , Poland. , (Poland)
  • 4 d Department of Biochemistry and Biotechnology , Poznan University of Life Sciences , Poznań , Poland. , (Poland)
  • 5 e Department of Animal Nutrition , The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences , Jabłonna , Poland. , (Poland)
  • 6 f Faculty of Geology , University of Warsaw , Warsaw , Poland. , (Poland)
  • 7 g Department of Astrophysics, Institute of Physics , Jan Kochanowski University in Kielce , Poland. , (Poland)
  • 8 h Faculty of Environmental, Geomatic and Energy Engineering , Kielce University of Technology , Kielce , Poland. , (Poland)
Type
Published Article
Journal
Biofouling
Publisher
Informa UK (Taylor & Francis)
Publication Date
Jan 01, 2019
Volume
35
Issue
1
Pages
59–74
Identifiers
DOI: 10.1080/08927014.2018.1564818
PMID: 30727772
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Pseudomonas aeruginosa biofilm-associated infections are a serious medical problem, and new compounds and therapies acting through novel mechanisms are much needed. Herein, the authors report a ruthenium(IV) complex that reduces P. aeruginosa PAO1 biofilm formation by 84%, and alters biofilm morphology and the living-to-dead cell ratio at 1 mM concentration. Including the compound in the culture medium altered the pigments secreted by PAO1, and fluorescence spectra revealed a decrease in pyoverdine. Scanning electron microscopy showed that the ruthenium complex did not penetrate the bacterial cell wall, but accumulated on external cell structures. Fluorescence quenching experiments indicated strong binding of the ruthenium complex to both plasmid DNA and bovine serum albumin. Formamidopyrimidine DNA N-glycosylase (Fpg) protein digestion of plasmid DNA isolated after ruthenium(IV) complex treatment revealed the generation of oxidative stress, which was further proved by the observed upregulation of catalase and superoxide dismutase gene expression.

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