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The beneficial effects of reduced magnesium during the oocyte-to-embryo transition are conserved in mice, domestic cats and humans.

Authors
  • Herrick, Jason R
  • Strauss, Kevin J
  • Schneiderman, Ann
  • Rawlins, Mary
  • Stevens, John
  • Schoolcraft, William B
  • Krisher, Rebecca L
Type
Published Article
Journal
Reproduction, fertility, and development
Publication Date
Jan 01, 2015
Volume
27
Issue
2
Pages
323–331
Identifiers
DOI: 10.1071/RD13268
PMID: 24280268
Source
Medline
License
Unknown

Abstract

In many cell types Mg2+ can antagonise Ca2+ -stimulated signalling pathways, but information regarding the effects of these ions on IVF and subsequent embryonic development is limited. Our objectives were to evaluate the effects of Mg2+ in the IVF medium on embryonic development in mice and then determine if similar effects occurred in domestic cats and humans. Oocytes from hybrid and outbred mice, domestic cats and humans were fertilised (IVF, mice and cats; intracytoplasmic sperm injection (ICSI), humans) in the presence of 0.2 or 1.2 (mouse and human) or 1.0 (cat) mM Mg2+ and the resulting embryos were cultured to the blastocyst stage. Decreased concentrations of Mg2+ during IVF increased (P<0.05) cleavage of oocytes from outbred mice (77.9 vs. 51.0%), development of embryos from hybrid mice (74.5 vs. 51.0% hatching blastocyst per cleaved embryo) and both cleavage (68.4 vs. 46.8%) and blastocyst development (53.0 vs. 26.2% per cleaved embryo) in cats. Development to the blastocyst stage (52.1 vs. 40.2%) was also improved (P<0.05) when ICSI was performed on human oocytes in the presence of 0.2 mM Mg2+, compared with a commercial culture medium. Sensitivity to increased (1.0 to 1.2 mM) concentrations of Mg2+ in the medium during the oocyte-to-embryo transition appears to be conserved in three different species.

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