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Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

Authors
  • Leinonen, Hanna M.1, 2
  • Lepola, Saana1, 2
  • Lipponen, Eevi M.1, 2
  • Heikura, Tommi3
  • Koponen, Tiina3
  • Parker, Nigel3
  • Ylä-Herttuala, Seppo3
  • Lesch, Hanna P.1, 2
  • 1 Kuopio Center for Gene and Cell Therapy, Kuopio, Finland
  • 2 FinVector, Kuopio, Finland; and
  • 3 Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
Type
Published Article
Journal
Human Gene Therapy
Publisher
Mary Ann Liebert
Publication Date
Mar 01, 2020
Volume
31
Issue
5-6
Pages
376–384
Identifiers
DOI: 10.1089/hum.2019.247
PMID: 32075423
PMCID: PMC7087403
Source
PubMed Central
Keywords
License
Green

Abstract

We have previously produced viral vectors (lentiviral vector, adenoviral vector, and adeno-associated viral vector) in small and in commercial scale in adherent cells using Pall fixed-bed iCELLis® bioreactor. Recently, a company called Univercells has launched a new fixed-bed bioreactor with the same cell growth surface matrix material, but with different fixed-bed structure than is used in iCELLis bioreactor. We sought to compare the new scale-X™ hydro bioreactor (2.4 m2) and iCELLis Nano system (2.67 m2) to see if the difference has any effect on cell growth or lentiviral vector and adenoviral vector productivity. Runs were performed using parameters optimized for viral vector production in iCELLis Nano bioreactor. Cell growth was monitored by counting nuclei, as well as by following glucose consumption and lactate production. In both bioreactor systems, cells grew well, and the cell distribution was found quite homogeneous in scale-X bioreactor. Univercells scale-X bioreactor was proven to be at least equally efficient or even improved in both lentiviral vector and adenoviral vector production. Based on the results, the same protocol and parameters used in viral vector production in iCELLis bioreactor can also be successfully used for the production in scale-X bioreactor system.

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