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Behavioral remodeling of normal and cancerous epithelial cell lines with differing invasion potential induced by substrate elastic modulus.

Authors
  • Ansardamavandi, Arian1
  • Tafazzoli-Shadpour, Mohammad1
  • Shokrgozar, Mohammad Ali2
  • 1 a Faculty of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran. , (Iran)
  • 2 b National Cell Bank of Iran (NCBI), Pasteur Institute of Iran , Tehran , Iran. , (Iran)
Type
Published Article
Journal
Cell Adhesion & Migration
Publisher
Landes Bioscience
Publication Date
Aug 28, 2018
Pages
1–17
Identifiers
DOI: 10.1080/19336918.2018.1475803
PMID: 29969940
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The micro-environment of cancer cells in the body is mechanically stiffer than that of normal cells. We cultured three breast cell lines of MCF10A-normal, MCF7-noninvasive, and MDA-MB-231-invasive on PDMS substrates with different elastic moduli and different cellular features were examined.Effects of substrate stiffness on cell behavior were evident among all cell lines. Cancerous cells were more sensitive to substrate stiffness for cell behaviors related to cell motility and migration which are necessary for invasion. The invasive cancerous cells were the most motile on substrates with moderate stiffness followed by non-invasive cancerous cells. Gene markers alterations were generally according to the analyzed cell movement parameters. Results suggest that alterations in matrix stiffness may be related to cancer disease and progression.

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