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The behavioral and molecular evaluation of effects of social instability stress as a model of stress-related disorders in adult female rats.

Authors
  • Nowacka-Chmielewska, Marta Maria1, 2
  • Kasprowska-Liśkiewicz, Daniela1, 2
  • Barski, Jarosław Jerzy2, 3
  • Obuchowicz, Ewa4
  • Małecki, Andrzej1
  • 1 a Laboratory of Molecular Biology, Faculty of Physiotherapy , The Jerzy Kukuczka Academy of Physical Education , Katowice , Poland. , (Poland)
  • 2 b Department of Experimental Medicine, School of Medicine in Katowice , Medical University of Silesia , Katowice , Poland. , (Poland)
  • 3 c Department of Physiology, School of Medicine in Katowice , Medical University of Silesia , Katowice , Poland. , (Poland)
  • 4 d Department of Pharmacology, School of Medicine in Katowice , Medical University of Silesia , Katowice , Poland. , (Poland)
Type
Published Article
Journal
Stress (Amsterdam, Netherlands)
Publication Date
Nov 01, 2017
Volume
20
Issue
6
Pages
549–561
Identifiers
DOI: 10.1080/10253890.2017.1376185
PMID: 28911267
Source
Medline
Keywords
License
Unknown

Abstract

The study aimed to test the hypotheses that chronic social instability stress (CSIS) alters behavioral and physiological parameters and expression of selected genes important for stress response and social behaviors. Adult female Sprague-Dawley rats were subjected to the 4-week CSIS procedure, which involves unpredictable rotation between phases of isolation and overcrowding. Behavioral analyses (Experiment 1) were performed on the same rats before and after CSIS (n = 16) and physiological and biochemical measurements (Experiment 2) were made on further control (CON; n = 7) and stressed groups (CSIS; n = 8). Behaviors in the open field test (locomotor and exploratory activities) and elevated-plus maze (anxiety-related behaviors) indicated anxiety after CSIS. CSIS did not alter the physiological parameters measured, i.e. body weight gain, regularity of estrous cycles, and circulating concentrations of stress hormones and sex steroids. QRT-PCR analysis of mRNA expression levels was performed on amygdala, hippocampus, prefrontal cortex (PFC), and hypothalamus. The main finding is that CSIS alters the mRNA levels for the studied genes in a region-specific manner. Hence, expression of POMC (pro-opiomelanocortin), AVPR1a (arginine vasopressin receptor), and OXTR (oxytocin receptor) significantly increased in the amygdala following CSIS, while in PFC and/or hypothalamus, POMC, AVPR1a, AVPR1b, OXTR, and ERβ (estrogen receptor beta) expression decreased. CSIS significantly reduced expression of CRH-R1 (corticotropin-releasing hormone receptor type 1) in the hippocampus. The directions of change in gene expression and the genes and regions affected indicate a molecular basis for the behavior changes. In conclusion, CSIS may be valuable for further analyzing the neurobiology of stress-related disorders in females.

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