Affordable Access

deepdyve-link
Publisher Website

Behavior of the IncP-7 carbazole-degradative plasmid pCAR1 in artificial environmental samples.

Authors
  • Shintani, Masaki
  • Matsui, Kazuhiro
  • Takemura, Tetsuo
  • Yamane, Hisakazu
  • Nojiri, Hideaki
Type
Published Article
Journal
Applied Microbiology and Biotechnology
Publisher
Springer-Verlag
Publication Date
Sep 01, 2008
Volume
80
Issue
3
Pages
485–497
Identifiers
DOI: 10.1007/s00253-008-1564-5
PMID: 18592232
Source
Medline
License
Unknown

Abstract

In artificial environmental samples, the behavior of the IncP-7 conjugative plasmid pCAR1, which is involved in the catabolism of carbazole, was monitored. Sterile soil and water samples supplemented with carbazole were prepared. After inoculation with Pseudomonas putida harboring pCAR1, seven species of the genus Pseudomonas, and three other bacterial species, were monitored for carbazole degradation, bacterial survival, and conjugative transfer of pCAR1. In artificial soils, more than 90% of the carbazole was degraded in samples with high water content, suggesting that the water content is a key factor in carbazole degradation in artificial soils. In three of the artificial environmental water samples, more than 95% of the carbazole was degraded. Transconjugants were detected in some artificial water samples, but not in the artificial soil samples, suggesting that pCAR1 is preferably transferred in aqueous environments. Composition analysis of the artificial water samples and examination of conjugative transfer indicated that the presence of the divalent cations Ca(2+) and Mg(2+) promoted the plasmid transfer. The presence of carbazole also increases in incidence of transconjugants, probably by enhancing their growth. In contrast, humic acids in the liquid layer of artificial soil samples appeared to prevent conjugative transfer.

Report this publication

Statistics

Seen <100 times