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Cytochrome P-450-catalyzed formation of 20-hydroxy-ecdysone in larval housefly mitochondria

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
166
Issue
3
Identifiers
DOI: 10.1016/0006-291x(90)91018-n

Abstract

Abstract Six forms of cytochrome P-450 in the mitochondria of larvae from Musca domestica were isolated by solubilization with CHAPS followed by ammonium sulfate fractionation and HPLC on an anion-exchange column. Forms 1, 2, 3, 5, and 6 catalyzed the formation of 20-hydroxy-ecdysone from ecdysone in the presence of NADPH and pig adrenal adrenodoxin and adrenodoxin reductase at rates not much different that observed in mitochondria; whereas, fraction 4 showed an activity which was about 10-fold higher than mitochondria. Forms 4 and 5 were further purified by HPLC on a cation-exchange column followed by removal of excess detergent by hydroxyl apatite column chromatography. In vitro reconstitution of the monooxygenase activity confirmed that form 4 is primarily involved in the formation of 20-hydroxy-ecdysone from ecdysone. SDS-polyacrylamide gel electrophoresis indicated a high degree of purity of both forms 4 and 5, with molecular weights of 56 and 58 KDa, respectively.

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